Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis

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Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis.

Two different synergy testing methods, the checkerboard and the E test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures. The rate of agreement of the E test and checkerboard methods was found to be 55%. The most concordant results were found for the streptomycin-doxycycline combination...

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In vitro synergistic activity of antibiotic combinations against Brucella melitensis using E-test methodology

The treatment of brucellosis is still problematic, because of high rates of treatment failure or relapses. As the microorganism is an intracellular pathogen, treatment requires combined regimens. However, limited existing data on in vitro combinations are avaliable for Brucellae. The aim of this study was to investigate the in vitro efficacy of various traditional and new antibiotic combination...

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Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test.

An in vitro method of detecting synergy which is simple to perform, accurate, and reproducible and has the potential for clinical extrapolation is desirable. Time-kill and checkerboard methods are the most widely used techniques to assess synergy but are time-consuming and labor-intensive. The Epsilometer test (E test), a less technically demanding test, has not been well studied for synergy te...

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Protection of BALB/c mice against pathogenic Brucella abortus and Brucella melitensis by vaccination with recombinant Omp16

Objective(s): Prevention of the globally spread zoonotic infection, brucellosis which affects an extensive range of hosts is still challenging researchers. There are no approved vaccines for the prevention of human disease and those used for animal brucellosis have adverse properties, which limit their application. We investigated the immunological and protective effec...

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Efficacy of several antibiotic combinations against Brucella melitensis Rev 1 experimental infection in BALB/c mice.

OBJECTIVES The objective of the present study was to compare the efficacy of gentamicin given alone or combined with doxycycline with that of standard combination therapies in BALB/c mice experimentally infected with the Brucella melitensis vaccine strain Rev 1. METHODS A standard broth microdilution method was applied to determine the susceptibility of strain Rev 1 to the clinically most rel...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2005

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.43.1.140-143.2005